English
norskBlar i forfatter "Ingebrigtsen, Richard Andre"
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Adding zooplankton to the OSMAC toolkit: Effect of grazing stress on the metabolic profile and bioactivity of a diatom
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-02-03)“One strain many compounds” (OSMAC) based approaches have been widely used in the search for bioactive compounds. Introducing stress factors like nutrient limitation, UV-light or cocultivation with competing organisms has successfully been used in prokaryote cultivation. It is known that diatom physiology is affected by changed cultivation conditions such as temperature, nutrient concentration and ... -
Chemical Diversity as a Function of Temperature in Six Northern Diatom Species
(Journal article; Tidsskriftartikkel; Peer reviewed, 2013)In this study, we investigate how metabolic fingerprints are related to temperature. Six common northern temperate diatoms (Attheya longicornis, Chaetoceros socialis, Chaetoceros furcellatus, Porosira glacialis, Skeletonema marinoi, and Thalassiosira gravida) were cultivated at two different temperatures, 0.5 and 8.5 °C. To exclude metabolic variations due to differences in growth rates, the growth ... -
The Effect of Freeze/Thaw Cycles on Reproducibility of Metabolic Profiling of Marine Microalgal Extracts Using Direct Infusion High-Resolution Mass Spectrometry (HR-MS)
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014-10-13)During normal sample preparation, storage in freezers and subsequent freeze/thaw cycles are commonly introduced. The effect of freeze/thaw cycles on the metabolic profiling of microalgal extracts using HR-MS was investigated. Methanolic extracts of monocultures of Arctic marine diatoms were analyzed immediately after extraction, after seven days of storage at −78 °C (one freeze/thaw cycle), and after ... -
Field sampling marine plankton for biodiscovery
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-11-20)Microalgae and plankton can be a rich source of bioactivity. However, induction of secondary metabolite production in lab conditions can be difficult. One simple way of bypassing this issue is to collect biomass in the field and screen for bioactivity. Therefore, bulk net samples from three areas along the coast of northern Norway and Spitsbergen were collected, extracted and fractionated. ... -
Inclusion of photoautotrophic cultivated diatom biomass in salmon feed can deter lice
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-29)The aim of this study was to evaluate the potential of diatom (microalgae) biomass as a lice-reducing ingredient in salmon feed. The original hypothesis was based on the fact that polyunsaturated aldehydes (PUAs), e.g. 2-trans, 4-trans decadenial (A3) produced by diatoms can function as grazing deterrents and harm copepod development. Salmon lice (Lepeophtheirus salmonis) is a copepod, and we intended ... -
Light and temperature effects on bioactivity in diatoms
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015-06-05)Isolates of five pelagic North Atlantic marine diatoms (Bacillariophyceae): Attheya longicornis, Chaetoceros socialis, Chaetoceros furcellatus, Skeletonema marinoi and Porosira glacialis were cultivated in large photobioreactors at two light and two temperature regimes to test if this affected bioactivity. We screened for bioactivity in assays representing five different therapeutic areas: ... -
Marine diatoms are a rich source of bioactivity
(Conference object; Konferansebidrag, 2014-11-03)As part of the MabCent-SFI drug-discovery initiative organized by the University of Tromsø, the coastal areas of northern Norway and the Barents Sea have been investigated (2006-2014). We saw that bioac- tive compounds from diatoms are very rarely reported (1-4). There- fore we desided that during the initial MabCent research cruises, di- atom strains should be isolated and kept in a culture collection ... -
Mass Cultivation of Microalgae: I. Experiences with Vertical Column Airlift Photobioreactors, Diatoms and CO2 Sequestration
(Journal article; Tidsskriftartikkel; Peer reviewed, 2022-03-17)From 2015 to 2021, we optimized mass cultivation of diatoms in our own developed vertical column airlift photobioreactors using natural and artificial light (LEDs). The project took place at the ferrosilicon producer Finnfjord AS in North Norway as a joint venture with UiT—The Arctic University of Norway. Small (0.1–6–14 m<sup>3</sup>) reactors were used for initial experiments and to produce inoculum ... -
Microbial Community Dynamics during a Harmful Chrysochromulina leadbeateri Bloom in Northern Norway
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-01-09)A harmful algal bloom occurred in late spring 2019 across multiple, interconnected fjords and bays in northern Norway. The event was caused by the haptophyte Chrysochromulina leadbeateri and led to severe fish mortality at several salmon aquaculture facilities. This study reports on the spatial and temporal succession dynamics of the holistic marine microbiome associated with this bloom by relating ...
